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Updated: Aug 17, 2026
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Published on: January 6, 2018
[Differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment]
Yu-tao Zhan1, Lai Wei, Hong-song Chen
1Hepatology Institute, People's Hospital, Peking University, Beijing 100044, China.
Objective:
To observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.
Methods:
Rat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.
Results:
The PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.
Conclusion:
Bone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts
