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A conserved signal-responsive sequence mediates activation-induced alternative splicing of CD45
Caryn Rothrock1, Brian Cannon, Brandon Hahm
1Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
Molecular Cell
|November 26, 2003
Summary
Researchers identified a specific DNA sequence element that controls alternative splicing in T cells stimulated by PMA. This element regulates gene expression and may be common to other signal-regulated genes.
Area of Science:
- Molecular Biology
- Cell Signaling
- Gene Regulation
Background:
- Alternative splicing is crucial for protein diversity and cellular responses to external signals.
- Understanding the regulatory sequences for signal-induced alternative splicing is key to identifying regulated genes.
Purpose of the Study:
- To identify and characterize the sequence element responsible for signal-induced alternative splicing.
- To determine if this element can confer activation-induced exon skipping in other genes.
Main Methods:
- Defined a specific sequence element involved in CD45 variable exon exclusion after T cell stimulation.
- Tested the sufficiency of this element in conferring activation-induced skipping of a heterologous exon.
Main Results:
- Identified a novel sequence element that dictates CD45 alternative splicing upon PMA stimulation.
- Demonstrated that this element is sufficient to induce exon skipping in a different gene context.
- Found homology of this regulatory element in other signal-regulated genes.
Conclusions:
- A specific sequence element is a primary determinant of signal-induced alternative splicing.
- This element's presence in multiple genes suggests shared regulatory pathways for alternative splicing across gene families.