Related Experiment Video
Updated: Aug 30, 2026

Subnanometer-Resolution Structural Determination of Hemagglutinin from Cryo-Electron Tomography of Influenza Viruses
Published on: November 7, 2025
Structure of an influenza neuraminidase-diabody complex by electron cryomicroscopy and image analysis
Peter B Rosenthal1, Lynne J Waddington, Peter J Hudson
1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK. rose@mrc-lmb.cam.ac.uk
Abstract:
The structure of a complex between a bivalent diabody and its antigen, influenza neuraminidase, has been determined by electron cryomicroscopy of single particles and image analysis. A three-dimensional reconstruction has been interpreted in terms of high-resolution X-ray models of the component proteins. The complex consists of two neuraminidase tetramers cross-linked by four diabodies with 422 point symmetry. The structure and symmetry of the complex is determined uniquely by packing constraints consistent with the maximum possible number of diabody cross-links. Diabodies may provide a useful approach to the structure determination of small proteins by incorporating the proteins into large symmetric complexes followed by single-particle electron microscopy.
Related Concept Videos
Cryo-electron Microscopy
Inhibitors Of Virion Release
Influenza

