A homogeneous high-throughput genotyping method based on competitive hybridization
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Updated: Aug 30, 2026
Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Minna Kiviniemi1, Jussi Nurmi, Hannu Turpeinen
1Department of Virology, University of Turku, Tykistökatu 6 A, 4th floor, 20520 Turku, Finland. minna.kiviniemi@utu.fi
Objectives:
A reliable high-throughput assay system is necessary for the analysis of the ever-increasing numbers of single-nucleotide polymorphisms (SNP) relevant to genetic screening studies. We describe an assay suitable also for large-scale screening programs.
Design And Methods:
The one-step assay is based on asymmetric PCR amplification of the target sequence and subsequent time-resolved fluorescence measurement. Asymmetric amplification results in a single-stranded PCR product that is detected in the amplification vessel with a highly sensitive, homogeneous hybridization method.
Results:
A dual label, homogeneous high-throughput platform for nucleic acid sequence analysis was developed and validated using a C/T single-nucleotide polymorphism in the insulin gene as a model analyte and applied also to two other SNP-assays (poliovirus receptor A/G-polymorphism and CD86-gene exon 2 A/G-polymorphism).
Conclusions:
The described high-throughput genotyping technology is very competitive in price, simple in design and easily applied to any analyte sequence.
