Influence of multiple substrates on anaerobic protein degradation in a packed-bed bioreactor

Data on the influence of substrate composition on the anaerobic degradation of bovine serum albumin (BSA) in a bench-scale packed-bed reactor are presented and discussed from the standpoint of substrate consumption kinetics. The experiments were carried out in a horizontal-flow anaerobic immobilized biomass (HAIB) reactor fed with BSA based substrates. BSA was the sole carbon source in the first one, while the others were composed of BSA, carbohydrates and lipids. In all the experiments, the HAIB reactor was operated at the hydraulic detention time of 4 hours. The reactor's performance was evaluated based on physicochemical and chromatographic analyses and also on microscopy techniques. A kinetic model of irreversible first-order series-parallel reactions with two intermediate products was proposed, allowing evaluation of the microbial consortium's affinity with the substrates and the metabolic compounds formed. As the first-order kinetic model adhered quite well to the experimental data, the initial protein degradation rates (k) were estimated. The presence of carbohydrates and lipids led the initial protein degradation rate to be reduced. However, the system fed with protein and carbohydrates showed higher process stability.