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Isolation and properties of major components of Penicillium canescens extracellular enzyme complex
O A Sinitsyna1, F E Bukhtoyarov, A V Gusakov
1Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia. oasinitsyna@enzyme.chem.msu.ru
Abstract:
The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with beta-galactosidase, the major components of the complex were endo-beta-1,4-xylanase (31 kD, pI 8.2-9.3), alpha-L-arabinofuranosidase (60 kD, pI 7.6), arabinoxylan-arabinofuranohydrolase (70 kD, pI 3.8-4.0), and endo-beta-1,3/1,4-glucanase (40 kD, pI 4.4). The substrate specificity, pH and temperature activity optima, adsorbability, thermal stability, and ability for synergic interaction of the isolated enzymes were studied.
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