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Updated: Jul 20, 2026
12:51
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 10, 2013
Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging
I Karakikes1, R E Barber, I E G Morrison
1Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, U.K.
Biochemical Society Transactions
|December 4, 2003
Abstract:
Dual-wavelength single-particle fluorescence imaging has been used to quantify the co-localization of receptors and/or ligands on cells by widefield microscopy. Methods for correction of chromatic aberration and identification of submicroscopic artefacts are presented, with data for the lipopolysaccharide/CD14 and MHC class II/CD74 systems.
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