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Related Experiment Videos

Mohs tissue mapping and processing: a survey study.

Sirunya Silapunt1, S Ray Peterson, Joseph Alcalay

  • 1DermSurgery Associates, 7515 Main Street, Suite 240, Houston, TX 77030, USA.

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]
|December 3, 2003
PubMed
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Mohs micrographic surgery (MMS) techniques for tissue mapping and processing show variability among practitioners. However, maintaining the integrity of each step ensures the high cure rates associated with this skin cancer treatment.

Area of Science:

  • Dermatology
  • Surgical Oncology
  • Pathology

Background:

  • Mohs micrographic surgery (MMS) is a precise surgical technique for treating skin cancer.
  • While the MMS concept is straightforward, its practical execution involves complex tissue handling procedures.

Purpose of the Study:

  • To investigate the diverse techniques used by members of the American College of Mohs Micrographic Surgery and Cutaneous Oncology.
  • Focus on current practices in Mohs tissue mapping and processing.

Main Methods:

  • A survey of 580 members of the American College of Mohs Micrographic Surgery and Cutaneous Oncology was conducted.
  • 310 responses (53%) were collected and analyzed regarding tissue mapping and processing methods.

Main Results:

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  • Most surgeons (66.5%) prepare tissue maps themselves, often using hand-drawn diagrams (69.4%).
  • Histotechnicians typically prepare specimens (63.5%), with heat extractors or tissue cuts used for flattening (52.9%).
  • Hematoxylin and eosin staining is prevalent (82.6%), and specimens are often sectioned into 3-6 slides at 5-6 micrometers.

Conclusions:

  • Significant variability exists in Mohs tissue mapping and processing techniques.
  • Preserving the integrity of each procedural step is crucial for maintaining the efficacy of Mohs surgery.