Related Experiment Video
Updated: Aug 30, 2026
Murine Model of CD40-activation of B cells
Published on: March 5, 2010
[Functional significance of CD40 and CD40 ligand linking on human lung cells]
1Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Objective:
To determine the possible functional significance of CD40 and CD40 ligand (CD154) linking on human lung carcinomas and to assess the potential of CD40 as a therapeutic target.
Methods:
We evaluated the effect of CD40L on the surface expression of major histocompatibility complex class I (MHC-I), Fas, bcl2, CD54, epidermal growth factor receptor (EGFR), p-glucoprotein (PGP), lung related protein (LRP), cell cycle, cell apoptosis and growth kinetics of 7 lung cancer cell lines (including 1 CD40-transfected cell line GLC-82/CD40) by gene cloning, Western blotting, and flow cytometry etc.
Results:
Significant increased expression of MHC-I, CD54 and Fas was observed in 4 tumor lines expressing high levels of CD40 after CD40L (0.1 micro g/ml) linked to CD40. CD40L (0.1 micro g/ml or greater) in the fifth day was found to significantly inhibit the proliferation of 4 cell lines expressing high levels of CD40, decreased the percentage of aneuploid cell, and inhibited S-phase cells entering G2/M phase. The effect of CD40 cross-linking was reversible. CD40-moderate and low and negative tumor did not respond to CD40L. All of 7 cell lines show no significant changes in apoptosis in either the experimental (CD40L pulsed) or control (medium only) cell cultures.
Conclusion:
Expression of CD40 on lung tumors cells expressing high level of CD40 (including CD40-transfected cells) may represent a potential therapeutic target.
