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[Expression of midkine and its relationship with HBV infection in hepatocellular carcinomas]
Li-cheng Dai1, Xing Yao, Yong-liang Lu
1Department of Infection, Huzhou Central Hospital, Huzhou 313000, China.
Objective:
To investigate expression of midkine (MK) in hepatocellular carcinoma (HCC) and its relationship with HBV infection.
Methods:
Expression of MK mRNA, MK protein and HBV DNA were determined in 62 cases of human HCC and 10 cases of controls with in situ hybridization and immunohistochemistry.
Results:
Positive rates of MK mRNA and MK protein in carcinoma tissue were 74.2% and 75.8% respectively, which were higher than in paratumorous liver tissue and normal controls (P < 0.01). Positive rate of HBV DNA in the nuclei of carcinoma tissue (62.9%) were higher than in paratumorous liver tissue (6.5%) (P < 0.01). There was a significant relationship between expression of MK mRNA and MK protein in the carcinoma tissue and HBV DNA in the nuclei of carcinoma tissue.
Conclusions:
HCC overexpresses MK at the mRNA and protein level. The overexpression of the MK protein might be correlated with the existence of HBV DNA in the nuclei of carcinoma tissue.
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