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[Study on the in vivo effect of rhG-CSF on peripheral T lymphocyte]
1Institute of Hematology & People's Hospital, Peking University, Beijing 100044, China.
Objective:
To investigate the mechanisms of in vivo rhG-CSF affecting T lymphocyte.
Methods:
Peripheral blood and apheresis graft were obtained from sibling donors before and after G-CSF administration. Proliferation of peripheral blood mononuclear cells (MNC) stimulated by PHA before and after removing monocyte by plastic-adherence was detected by MTT test. Absolute number of MNC, costimulating molecules expression on antigen presenting cells (monocyte and B lymphocyte) and the number of CD(14)(+) cells before and after plastic-adherence treatment were analyzed by flow cytometry.
Results:
After rhG-CSF administration, absolute peripheral blood monocyte counts increased by 4.2 +/- 1.74 times. By removing monocytes, proliferation of T cells after rhG-CSF administration partly restored, but remained 20.58% lower than that before G-CSF treatment. CD(86) co-stimulating factor expression on monocyte declined (66.96 +/- 13.87)% and mean fluorescence intensity (MIF) decreased (31.31 +/- 12.91)% after rhG-CSF administration. Expression of CD(80) on B lymphocyte decreased (45.77 +/- 26.58)%.
Conclusions:
The increased number of monocytes after rhG-CSF administration suppressed T-cell proliferation, which was partly the mechanism of the T-cell hyporesponsiveness. The fact that monocyte and B lymphocyte expressed low level of B(7) co-stimulating factor suggested that antigen present cells might also mediate the alteration of T-cell proliferation.
Insights
Recombinant human granulocyte-colony stimulating factor (rhG-CSF) increases monocytes, suppressing T-lymphocyte proliferation. This study reveals rhG-CSF alters T-cell responses by affecting monocyte and B-lymphocyte co-stimulatory molecule expression.
Area of Science:
- Immunology
- Hematology
Context:
- Granulocyte-colony stimulating factor (G-CSF) is widely used to mobilize hematopoietic stem cells.
- The impact of G-CSF on T-lymphocyte function remains incompletely understood.
- Investigating G-CSF's effects on T-cell proliferation and antigen-presenting cells is crucial for optimizing cell therapies.
Purpose:
- To elucidate the mechanisms by which recombinant human G-CSF (rhG-CSF) influences T-lymphocyte function in vivo.
- To assess the changes in T-cell proliferation and the expression of co-stimulatory molecules on antigen-presenting cells following rhG-CSF administration.
Summary:
- rhG-CSF administration led to a significant increase in peripheral blood monocyte counts.
- T-cell proliferation was suppressed after rhG-CSF treatment, and this suppression was partially restored upon monocyte removal.
- Expression of co-stimulatory molecules CD86 on monocytes and CD80 on B lymphocytes decreased post-rhG-CSF treatment.
Impact:
- The findings suggest that increased monocyte numbers and altered co-stimulatory molecule expression on monocytes and B lymphocytes contribute to rhG-CSF-induced T-cell hyporesponsiveness.
- This research provides insights into the immunomodulatory effects of rhG-CSF, potentially impacting stem cell transplantation protocols.
- Understanding these mechanisms can inform strategies to mitigate adverse immune effects associated with G-CSF therapy.
