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Updated: Jul 10, 2026
Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
Published on: October 16, 2014
FT-IR study of heterologous protein expression in recombinant Escherichia coli strains
Diletta Ami1, Loredana Bonecchi, Simona Calì
1INFM UdR Milano-Bicocca, Department of Biotechnologies and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
Abstract:
Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation.
