Electrical breakdown of human erythrocytes: a technique for the study of electro-haemolysis

This paper describes a technique suitable for investigating the electromechanical breakdown properties of erythrocyte cells. The cells were exposed to square wave electric pulses of precise duration and voltage. The erythrocytes were suspended in normal isotonic saline between two opposing platinum electrodes. A red LED light source and photodiode detector system were positioned orthogonally to the electrodes to record changes in the light transmission that occur immediately after applying an electric pulse. The light transmitted through the electrically treated erythrocyte suspension could be monitored continuously. Experiments were conducted to explore the inter-relationship between the critical voltage and pulse length for haemolysis. Human blood taken from "healthy" donors underwent haemolysis at a critical field strength of 304 kV/m for a 5 micros pulse and 292 kV/m for a 50 micros pulse. The relationship of critical pulse length and critical voltage for the blood samples was found to be inversely linear.