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Updated: Aug 30, 2026

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
Published on: December 19, 2025
Application of reproductive biotechnology in animals: implications and potentials. Applications of reproductive
Lesley Paterson1, Paul DeSousa, William Ritchie
1Roslin Institute, Roslin, Midlothian, Scotland EH25 9PS, UK.
Abstract:
The development of new methods of nuclear transfer in mammals is creating many new opportunities in research, medicine and agriculture. The method of cloning is repeatable and has been established in many laboratories worldwide. However, the present procedure is inefficient with fewer than 4% of embryos becoming viable offspring. A considerable improvement in efficiency is required before wide scale use for livestock improvement. The opportunity to introduce precise genetic changes to livestock is available for the first time through the use of gene targeting procedures in cultured cells that are used as nuclear donors. This has potential application in the production of organs for transplantation to humans, studies of human genetic disease and basic research in to the control of gene expression and function.
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