Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aspergillus nidulans hypA regulates morphogenesis through the secretion pathway.

Xianzong Shi1, Yu Sha, Susan Kaminskyj

  • 1Department of Biology, University of Saskatchewan, SK, S7N 5E2 Saskatoon, Canada.

Fungal Genetics and Biology : FG & B
|December 4, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic and proteomic analysis of osteogenesis imperfecta disease-specific induced pluripotent stem cells.

BMC medical genomics·2026
Same author

Construction of a scaffold-mediated dual-enzyme display system in Komagataella phaffii with immobilized cells for continuous biocatalysis.

Bioresource technology·2026
Same author

Electronic-Structure-Regulated Hydrogen Atom Transfer for the Construction of High-Performance Nitroimino-Functionalized Fused Heterocyclic Energetic Materials.

Organic letters·2026
Same author

Stabilizing High-Energy Materials via a C-C-Connected [5,5]-Fused Triazolotriazole and Triazine Scaffold.

The Journal of organic chemistry·2026
Same author

Engineering Escherichia coli cell Factories for continuous 5'-cytidine monophosphate production via biofilm-anchored dual-enzyme cascade catalysis.

Bioresource technology·2026
Same author

Association of plasma remnant cholesterol with cognitive function in the middle-aged and elderly Chinese adults with type 2 diabetes: a cross-sectional study.

Frontiers in nutrition·2026

The Aspergillus nidulans hypA gene is crucial for hyphal growth and cell wall integrity. Mutations in hypA lead to altered cell morphology and reduced enzyme secretion, particularly at higher temperatures.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Mycology

Background:

  • The Aspergillus nidulans hypA gene encodes a large cytoplasmic peptide.
  • Specific alleles (hypA1, hypA6) cause distinct morphological changes at elevated temperatures.

Purpose of the Study:

  • To investigate the function of Aspergillus nidulans hypA.
  • To characterize the cellular defects associated with hypA mutations.

Main Methods:

  • Genetic analysis of hypA alleles and deletion strains.
  • Microscopic examination of hyphal morphology and cell wall structure.
  • Assessment of enzyme secretion and cytoskeletal organization.

Main Results:

  • hypA mutants exhibit wide, slow-growing hyphae with thick walls at 42°C.

Related Experiment Videos

  • hypA deletion strains require osmotic support and show aberrant morphology.
  • Enzyme secretion is reduced in hypA1 cells at 42°C, with impaired endomembrane and actin polarity, and reduced microtubules.

    • Conclusions:

    • The hypA gene product is essential for maintaining normal hyphal growth, cell wall integrity, and secretion pathways in Aspergillus nidulans.
    • hypA orthologs, like Saccharomyces cerevisiae TRS120, suggest a conserved role in intracellular trafficking and cellular organization.