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Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage
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Digoxin antibody prevents cerebral hemorrhage-induced hypertension

João C Menezes1, Varujan Dichtchekenian

  • 1Basic Research Laboratory, Nephrology (LIM-12), Medicine School, Sao Paulo University, Sao Paulo, Brazil.

American Journal of Hypertension
|December 4, 2003
PubMed

Insights

Intracerebroventricular hemorrhage increases blood pressure and serum ouabain-like compound (OLC). Digoxin antibody effectively reversed these hypertension effects in a rat model.

Area of Science:

  • Neuroscience
  • Cardiovascular Physiology

Background:

  • Brain injury can lead to hypertension.
  • Serum ouabain-like compound (OLC) possesses vasoconstrictor properties.
  • Investigating the antihypertensive potential of digoxin antibody is crucial.

Purpose of the Study:

  • To evaluate the antihypertensive effects of digoxin antibody.
  • To assess the role of serum OLC in hypertension induced by intracerebroventricular (ICV) hemorrhage.

Main Methods:

  • Wistar rats were divided into four ICV infused groups: control, blood, blood plus digoxin antibody, and cerebrospinal fluid-like solution.
  • Measurements included tail-cuff blood pressure, cumulative sodium balance, and serum OLC levels.
  • An intracerebroventricular hemorrhage rat model was utilized.

Main Results:

  • ICV blood infusion significantly increased blood pressure (BP) and serum OLC without altering sodium balance.
  • Administration of digoxin antibody successfully prevented the rise in BP and OLC.
  • A positive correlation was observed between BP and OLC in relevant rat groups (R = 0.63; P <.05).

Conclusions:

  • Cerebral hemorrhage is associated with increased OLC levels and elevated BP.
  • Digoxin antibody administration demonstrated a reversal of OLC and BP increases.
  • These findings suggest a therapeutic role for digoxin antibody in managing hypertension post-cerebral hemorrhage.
Abstract

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