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Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
Decolorization of molasses wastewater by a strain No.BP103 of acetogenic bacteria
Suntud Sirianuntapiboon1, Phimphaka Phothilangka, Sadahiro Ohmomo
1Department of Environmental Technology, School of Energy and Materials, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand. suntud.sir@kmutt.ac.th
Abstract:
Among 170 strains of acetogenic bacteria, a strain No.BP103 showed the highest decolorization yield (76.4+/-3.2%) when cultivated at 30 degrees C for 5 days in molasses pigments medium containing glucose 3.0%, yeast extract 0.5%, KH2PO4 0.1% and MgSO4 x 7H2O 0.05% and the pH adjusted to 6.0. In addition this strain could decolorize 32.3+/-3.2% and 73.5+/-3.5% of molasses pigments in stillage and anaerobically treated molasses wastewater, both supplementing glucose 3.0%, yeast extract 0.5%, KH2PO4 0.1% and MgSO4 x 7H2O 0.05%. However without nutrients supplement, the decolorization yields were 9.75+/-3.0% and 44.36+/-3.4%, respectively. In a replacement culture system involving six times replacement (30 days), the strain No.BP103 showed a constant decolorization yield of 72.0+/-3.2-84.0+/-3.2% and caused decreases of biological oxygen demand and chemical oxygen demand values of approximately 58.5+/-6.4-82.2+/-7.1% and 35.5+/-0.42-71.20.58%, respectively. Under a periodical feeding system, the decolorization yield was 30.0+/-2.1-45.0+/-3.5%.
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