Optimization of cyclodextrin production from sago starch

Area of Science:

• Biotechnology
• Enzymology
• Carbohydrate Chemistry

Background:

• Cyclodextrins (CDs) are versatile cyclic oligosaccharides produced by bacterial cyclodextrin glycosyltransferase (CGTase).
• CDs find extensive applications across food, pharmaceutical, cosmetic, and agricultural sectors.
• Optimizing CGTase activity is crucial for efficient CD production.

Purpose of the Study:

• To partially purify Bacillus circulans CGTase.
• To determine optimal conditions for cyclodextrin production from sago starch.
• To identify the ideal enzyme-to-substrate ratio for beta-cyclodextrin (β-CD) synthesis.

Main Methods:

• Partial purification of CGTase using ammonium sulfate precipitation (50-70% saturation).
• Determination of optimal pH (4.5-5.0) and temperature (55-60°C) for CD production.
• Response surface methodology applied to optimize the enzyme:substrate ratio.

Main Results:

• Partially purified CGTase exhibited optimal activity within pH 4.5-5.0 and 55-60°C.
• Beta-cyclodextrin (β-CD) was the primary product, comprising 65% of total CDs.
• No significant difference in β-CD yield or productivity was observed between crude and partially purified CGTase.
• The optimal enzyme:substrate ratio was determined as 50 U/g sago starch for CGTase and 60 g/L for sago starch.

Conclusions:

• Partial purification of Bacillus circulans CGTase is feasible and effective for CD production.
• Optimal conditions for β-CD synthesis from sago starch were established.
• The study provides a precise enzyme:substrate ratio for maximizing β-CD yield, enhancing industrial applicability.