Increase in hepatic mRNA expression of coagulant factors in type 2 diabetic model mice

The purpose of the study is to investigate whether hypercoagulation in diabetes is observed not only in increased plasma levels of the coagulative factors but also in increased hepatic mRNA levels.

The age-related changes of coagulation factors were determined using KK and KK-A(y) mice as a model for human type 2 diabetes mellitus. Expression of the alpha-, beta- and gamma-chains of fibrinogen-mRNA and prothrombin-mRNA from the mouse liver was examined by reverse transcription-polymerase chain reaction (RT-PCR).

Hemoglobin A(1c) (HbA(1C)), plasma fibrinogen, triglyceride and insulin levels increased apparently, especially in KK-A(y) mouse at 4 months old. The mRNA levels of gamma-chain of fibrinogen significantly increased at 3 and 4 months of age in both mice. The mRNA levels of alpha- and beta-chains of fibrinogen and prothrombin were significantly increased in 4-month-old KK-A(y) mouse.

These results suggest that the increase in hepatic mRNA expression of coagulant factors contributes to the hypercoagulable state in type 2 diabetic mice.