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Initial RNA expression in human monocytes after multiple injury: a screening pilot study on potentially
Peter Biberthaler1, P Neth, B Bach
1Chirurgische Klinik Innenstadt, Ludwig-Maximilians-Universität München, Nussbaumstrasse 20, D-81366 Munich, Germany. pbiberth@helios.med.uni-muenchen.de
European Journal of Medical Research
|December 4, 2003
Summary
In multiple injured patients, gene expression in monocytes showed minimal changes post-trauma. This study found few influenced genes and no correlation with trauma severity or multiple organ failure (MOF).
Area of Science:
- Immunology
- Genetics
- Trauma Medicine
Background:
- Immune system dysfunction initiates multiple organ failure (MOF) in trauma patients.
- Intracellular mechanisms, like monocyte mRNA expression, are poorly understood.
- Screening gene expression in monocytes can reveal trauma-related molecular changes.
Purpose of the Study:
- To screen mRNA expression patterns in circulating monocytes of multiple injured patients.
- To compare gene expression profiles with the clinical course and MOF score.
- To identify early molecular responses to severe traumatic injury.
Main Methods:
- Prospective pilot study of 6 blunt multiple injury patients (ISS 16-57).
- Monocytes isolated via CD14 magnetic cell sorting at 6, 12, 24, and 48 hours post-trauma.
- Human microarray analysis (1176 genes) and cluster analysis of gene expression alterations.
Main Results:
- Only 86 genes (approx. 5%) showed significant expression changes.
- High inter-individual variability observed in gene expression, except for housekeeping genes.
- No 'mediator burst' of classical pro- or anti-inflammatory cascades detected.
Conclusions:
- This is the first screening of mRNA expression in monocytes of multiple injured patients.
- Few genes are significantly influenced by traumatic events.
- No correlation found between gene expression changes and trauma severity or MOF development.