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Related Experiment Videos

Plasma membrane aquaporin activity can affect the rate of apoptosis but is inhibited after apoptotic volume decrease.

Elizabeth M Jablonski1, Ashley N Webb, Nisha A McConnell

  • 1Department of Biology, University of North Carolina, Charlotte, NC 28223, USA.

American Journal of Physiology. Cell Physiology
|December 3, 2003
PubMed
Summary

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Aquaporins (AQPs) are crucial for the apoptotic volume decrease (AVD), a key cell death event. Inhibiting AQPs blocks AVD and subsequent apoptosis, highlighting their role in regulating cell volume and programmed cell death.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • Apoptosis, or programmed cell death, involves a series of morphological changes, starting with apoptotic volume decrease (AVD).
  • Aquaporins (AQPs) are integral membrane proteins facilitating water transport across cell membranes.
  • The precise role of AQPs in the initiation and progression of apoptosis remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of aquaporins (AQPs) in the apoptotic volume decrease (AVD).
  • To determine how AQP activity influences downstream apoptotic events.
  • To explore the regulation and localization of AQPs during apoptosis.

Main Methods:

  • Inhibition of AQPs in ovarian granulosa cells and thymocytes.
  • Overexpression of AQP-1 in Chinese hamster ovary (CHO-AQP-1) cells.

Related Experiment Videos

  • Assays to measure cell volume, water permeability, mitochondrial membrane potential, and caspase-3 activation.
  • Immunofluorescence to assess AQP-1 localization in relation to TNF-R1.

    • Main Results:

    • Inhibition of AQPs blocked AVD and downstream apoptotic events, including cell shrinkage and DNA degradation.
    • Overexpression of AQP-1 accelerated apoptosis in CHO cells.
    • Apoptotic cells exhibited significantly lower water permeability compared to non-apoptotic cells.
    • AQP-1 colocalized with TNF-R1 on the cell membrane before and after AVD, suggesting stability.

    Conclusions:

    • AQP-mediated water loss is essential for the AVD and subsequent apoptotic processes.
    • Plasma membrane water permeability, regulated by AQPs, controls the rate of apoptosis.
    • Inactivation of AQPs post-AVD may facilitate the low intracellular K+ levels required for apoptotic enzyme activation.
    • AQP inactivation during apoptosis does not involve degradation or removal from the cell membrane.