Related Experiment Video

Updated: Aug 30, 2026

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
07:47

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker

Published on: September 15, 2023

CD200 is a novel p53-target gene involved in apoptosis-associated immune tolerance

Michael D Rosenblum1, Edit Olasz, Jeffery E Woodliff

  • 1Department of Pediatrics, Medical College of Wisconsin, Milwaukee 53226, USA.

Blood
|December 3, 2003
PubMed

Insights

The immune system must control responses to self-antigens released during cell death to prevent autoimmunity. This study reveals that CD200 protein expression increases on dying dendritic cells (DCs), suppressing immune responses to self-antigens.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Apoptosis (programmed cell death) generates self-antigens that can trigger autoimmune responses.
  • Mechanisms controlling immune tolerance to self-antigens during cell turnover are not fully understood.
  • Dendritic cells (DCs) play a critical role in initiating immune responses and maintaining self-tolerance.

Purpose of the Study:

  • To investigate the molecular mechanisms regulating immune responses to apoptosis-associated self-antigens.
  • To identify novel immunoregulatory molecules involved in maintaining self-tolerance during cell death.
  • To elucidate the role of CD200 in the immune response to apoptotic cells.

Main Methods:

  • Analysis of CD200 protein expression in murine dendritic cells (DCs) undergoing apoptosis.
  • Identification of regulatory pathways (p53, caspase-dependent) controlling CD200 expression during apoptosis.
  • In vitro assays measuring proinflammatory cytokine production in response to self-antigens.
  • In vivo studies assessing UVB-mediated tolerance to haptenated self-proteins.

Main Results:

  • CD200 expression is upregulated on murine DCs during apoptosis.
  • CD200 is identified as a p53-target gene, with its expression regulated by p53- and caspase-dependent pathways.
  • CD200 on apoptotic DCs suppresses proinflammatory cytokine production in vitro.
  • CD200 is essential for UVB-induced tolerance to haptenated self-proteins in vivo.

Conclusions:

  • Upregulation of CD200 on apoptotic DCs is a novel mechanism for suppressing immune reactivity to self-antigens.
  • This CD200-mediated suppression is crucial for maintaining self-tolerance under steady-state conditions.
  • Understanding these pathways may offer therapeutic targets for autoimmune diseases.

More Related Videos

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

Co-Culture In Vitro Systems to Reproduce the Cancer-Immunity Cycle12:19

Co-Culture In Vitro Systems to Reproduce the Cancer-Immunity Cycle

Published on: June 7, 2024

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...