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Related Experiment Videos

Decrease of H2O2 plasma membrane permeability during adaptation to H2O2 in Saccharomyces cerevisiae.

Miguel R Branco1, H Susana Marinho, Luisa Cyrne

  • 1Grupo de Bioquímica dos Oxidantes e Antioxidantes, Centro de Química e Bioquímica, Universidade de Lisboa, Portugal.

The Journal of Biological Chemistry
|December 3, 2003
PubMed
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Cells actively regulate plasma membrane permeability to hydrogen peroxide (H2O2) in response to oxidative stress. This adaptation enhances H2O2 resistance, revealing a novel cellular defense mechanism.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Oxidative Stress Research

Background:

  • Hydrogen peroxide (H2O2) was previously thought to freely diffuse across biomembranes.
  • Antioxidant enzymes can create H2O2 gradients when H2O2 is produced in a different cellular compartment.
  • The role of membrane permeability regulation in cellular response to oxidative stress remains largely unexplored.

Purpose of the Study:

  • To investigate whether cells actively regulate plasma membrane permeability to H2O2 as a response to oxidative stress.
  • To determine the relationship between H2O2 gradients, cellular resistance to H2O2, and intracellular H2O2 removal capacity.
  • To identify genetic factors influencing H2O2 permeability and sensitivity.

Main Methods:

  • Utilized Saccharomyces cerevisiae as a model organism.

Related Experiment Videos

  • Measured H2O2 gradients across the plasma membrane upon exposure to external H2O2.
  • Assessed cellular resistance to H2O2 and intracellular H2O2 removal capacity.
  • Investigated the effect of pre-exposure to H2O2 and cycloheximide on plasma membrane permeability.
  • Examined H2O2 permeability and sensitivity in ergosterol biosynthesis mutants (erg3Δ and erg6Δ).

    • Main Results:

    • H2O2 gradients across the plasma membrane were formed in yeast cells exposed to external H2O2.
    • A correlation was observed between the magnitude of H2O2 gradients and cellular resistance to H2O2.
    • No correlation was found between intracellular H2O2 removal capacity and H2O2 resistance.
    • Plasma membrane permeability to H2O2 decreased twofold in cells adapted to H2O2 or treated with cycloheximide.
    • Ergosterol biosynthesis mutants exhibited increased plasma membrane permeability to H2O2 and higher sensitivity.

    Conclusions:

    • The regulation of plasma membrane permeability to H2O2 is a newly identified mechanism for cellular adaptation to oxidative stress.
    • This adaptive response influences cellular redox compartmentalization.
    • Findings have implications for understanding the origin and evolution of eukaryotic cells.