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Related Experiment Videos

A stem structure in fibroblast growth factor receptor 2 transcripts mediates cell-type-specific splicing by

Andrew P Baraniak1, Erika L Lasda, Eric J Wagner

  • 1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.

Molecular and Cellular Biology
|December 4, 2003
PubMed
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Alternative splicing of fibroblast growth factor receptor 2 (FGFR2) is regulated by intronic elements. The study reveals the FGFR2 splicing stem-loop structure

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Splicing

Background:

  • Alternative splicing of fibroblast growth factor receptor 2 (FGFR2) pre-mRNA is cell-type-specific, utilizing exon IIIb in epithelial cells and exon IIIc in mesenchymal cells.
  • Epithelium-specific exon IIIb activation and exon IIIc repression are controlled by intronic activating sequence 2 (IAS2) and intronic splicing activator and repressor (ISAR) elements.
  • A stem structure formed by IAS2 and the ISAR core has been proposed to regulate FGFR2 alternative splicing.

Purpose of the Study:

  • To investigate the role of the IAS2-ISAR stem structure in the cell-type-specific alternative splicing of FGFR2.
  • To determine if the stem-forming capacity or the specific sequences of IAS2 and ISAR are critical for regulating exon IIIb inclusion.

Main Methods:

  • Functional analysis of FGFR2 pre-mRNA splicing in epithelial cells.

Related Experiment Videos

  • Replacement of native IAS2 and ISAR core sequences with unrelated stem-forming sequences.
  • Deletion of the IAS2-ISAR stem-loop region and juxtaposition of flanking sequences.
  • Identification of conserved elements required for exon IIIb activation.

    • Main Results:

    • Replacement of IAS2 and ISAR core with random stem-forming sequences maintained proper exon IIIb activation and exon IIIc repression.
    • Deletion of the stem-loop region and direct approximation of upstream and downstream sequences also preserved cell-type-specific splicing.
    • A conserved GCAUG element downstream of the ISAR core was identified as essential for efficient exon IIIb activation.

    Conclusions:

    • The IAS2-ISAR stem structure is dispensable for cell-type-specific FGFR2 exon IIIb activation.
    • The primary function of the IAS2-ISAR stem is to facilitate the approximation of regulatory sequences.
    • A conserved downstream GCAUG element is crucial for mediating exon IIIb activation in epithelial cells.