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Degradation of ferric EDTA by Burkhol cepacia
Hung-Yuan Fang1, Shih-Chin Chen, Szu-Lin Chen
1Graduate of Engineering Science, Technology School (Doctoral Program), National YunLin University of Science and Technology, Touliu, Yunlin 640, Taiwan. fanghy@es.yuntech.edu.tw
Abstract:
EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms--Agrobacterium, Gram-negative BNCI, and DSM9103--that can degrade EDTA and are published in the literature. We have successfully isolated a bacterial strain that can degrade EDTA. It was identified as Burkhol cepacia, an aerobic species, elliptically shaped with a length of 5-15 microm. The growth medium contains 1000 mg/L of ferric-EDTA as carbon source, 750 mg/L of (NH4)2SO4 + (NH2)2CO as nitrogen source, and 1000 mg/L of KH2PO4 as phosphorus source, and mineral factors such as Fe and Mg. Incubated at pH 7.0, 30 degrees C, and 150 rpm on a shaker for 15 d, the average specific growth rate of this microbe is 0.135 d-1, which shows that the respective degradation efficiency of Fe-EDTA and Cu-EDTA is 90 and 75% individually.
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