Structural analyses of Phycodnaviridae and Iridoviridae

Alan A Simpson¹, Narayanasamy Nandhagopal, James L Van Etten

¹Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907-2054, USA.

Acta Crystallographica. Section D, Biological Crystallography

|December 4, 2003

Summary

Large dsDNA viruses like Phycodnaviridae and Iridoviridae utilize pseudo-hexameric trimers instead of hexameric capsomers. This structural adaptation influences their assembly and interactions within icosahedral shells.

Area of Science:

Structural virology
Molecular biology
Biophysics

Background:

Icosahedral viruses, including Phycodnaviridae and Iridoviridae, assemble from capsomers arranged in trigonal arrays.
The Caspar and Klug theory predicted icosahedral virus assembly from hexameric capsomers.
Evolutionary gene duplication led some viruses to replace hexamers with pseudo-hexameric trimers.

Purpose of the Study:

To investigate the structural organization of large dsDNA viruses.
To analyze the interactions between pseudo-hexameric trimers in viral capsids.
To refine the fitting of atomic structures into cryo-electron microscopy reconstructions.

Main Methods:

Analysis of viral capsid structures using cryo-electron microscopy (cryoEM).
Characterization of protein-protein interactions between viral capsomers.
Computational modeling for fitting atomic structures into cryoEM data.

Main Results:

Large dsDNA viruses feature pseudo-hexameric trimers organized into pentasymmetrons and trisymmetrons.
Three distinct classes of interactions exist between trimeric capsomers.
Understanding these interactions aids in precise atomic structure fitting into cryoEM reconstructions.

Conclusions:

Pseudo-hexameric capsomers represent a distinct subset within the Caspar and Klug lattice framework.
The study provides insights into the evolution and assembly of large icosahedral viruses.
Accurate structural data enhances our understanding of viral architecture and assembly mechanisms.

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Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

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