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Updated: Aug 30, 2026

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
Published on: February 21, 2019
Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2
Eugenia Pechkova1, Giuseppe Zanotti, Claudio Nicolini
1Fondazione E.L.B.A., Rome, Italy.
Abstract:
The three-dimensional crystal structure of the triple-point mutant of the catalytic subunit of human protein kinase CK2alpha has been determined at 2.4 A resolution. Microcrystals of mutant CK2 catalytic subunit were obtained by a protein-crystallization method based on thin-film nanotechnology. These microcrystals (of about 20 micro m in diameter) were used for diffraction data collection by means of the microfocus beamline at the ESRF synchrotron. A comparison between the human protein kinase CK2alpha and the corresponding enzyme from a lower organism (Zea mays) is made.
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