Related Experiment Video
Updated: Aug 30, 2026
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
Crystallization of [Fe3S4]-ferredoxin from the hyperthermophile archaeon Pyrococcus furiosus
Michael S Nielsen1, Pernille Harris, Hans E M Christensen
1Department of Chemistry, Building 207, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Abstract:
Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 A, beta = 103.86 degrees, and diffract to 1.5 A resolution.
More Related Videos
Related Concept Videos
Acid Mine Drainage
Microbes and Other Elemental Cycles

