Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modified microbatch and seeding in protein crystallization experiments.

Allan D'Arcy1, Aengus Mac Sweeney, Alexander Habera

  • 1Morphochem A.G Basel, Switzerland. allen.darcy@morphochem.ch

Journal of Synchrotron Radiation
|December 4, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The CASP 16 Experimental Protein-Ligand Datasets.

Proteins·2025
Same author

Cellular Potency Optimization of Novel Heme-Binding Imidazo[5,1-<i>b</i>]thiazoles, Imidazo[1,5-<i>a</i>]pyridines and Pyrazines as Potent IDO1 Inhibitors Devoid of Cytochrome P450 Inhibition.

Journal of medicinal chemistry·2025
Same author

Accelerating the Hit-To-Lead Optimization of a SARS-CoV-2 Mpro Inhibitor Series by Combining High-Throughput Medicinal Chemistry and Computational Simulations.

Journal of medicinal chemistry·2025
Same author

Discovery of Galactopyranose-1-carboxamides as a New Class of Small, Novel, Potent, Selective, and Orally Active Galectin-3 Inhibitors.

ChemMedChem·2025
Same author

Discovery and binding mode of small molecule inhibitors of the apo form of human TDO2.

Scientific reports·2024
Same author

Identification of SARS-CoV-2 Mpro inhibitors through deep reinforcement learning for <i>de novo</i> drug design and computational chemistry approaches.

RSC medicinal chemistry·2024

Protein crystallization requires forming critical-sized nuclei. This study explores increasing protein concentration and using heterogeneous seeding to achieve nucleation for better crystal formation.

Area of Science:

  • Biochemistry and structural biology
  • Crystallography and materials science

Background:

  • Protein crystallization is crucial for determining protein structure.
  • Nucleation, the initial step in protein crystallization, requires achieving a critical aggregate size.
  • Many crystallization experiments fail due to insufficient supersaturation levels, hindering nucleation.

Purpose of the Study:

  • To investigate methods for overcoming nucleation challenges in protein crystallization.
  • To enable crystal formation at lower protein and precipitant concentrations.

Main Methods:

  • Utilizing a modified microbatch method to increase protein and precipitating agent concentrations.
  • Employing heterogeneous seeding techniques to influence the nucleation event.

Related Experiment Videos

Main Results:

  • The modified microbatch method facilitates achieving high supersaturation levels for spontaneous nucleation.
  • Heterogeneous seeding provides a means to induce crystallization at reduced concentrations.

Conclusions:

  • Both increased concentration strategies and seeding are effective for promoting protein nucleation.
  • These methods enhance the success rate of protein crystallization experiments.