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Proteome analysis of wheat lemma.

Sun-Hee Woo1, Makoto Kimura, Arisa Higa-Nishiyama

  • 1Laboratory for Remediation Research, Plant Science Center, RIKEN, Wako, Saitama, Japan.

Bioscience, Biotechnology, and Biochemistry
|December 4, 2003
PubMed
Summary

This study characterizes wheat lemma proteomes, revealing proteins involved in cellular metabolism and fewer N-terminal blocks compared to rice. These findings advance our understanding of wheat protein expression.

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Area of Science:

  • Proteomics
  • Plant Biology
  • Biochemistry

Background:

  • The proteome of wheat lemma (flowering stage) has not been previously characterized.
  • Understanding plant protein expression is crucial for crop improvement and functional genomics.

Purpose of the Study:

  • To construct and analyze the proteome of wheat lemma at the anthesis stage.
  • To compare wheat lemma protein characteristics with other plant species, specifically rice.
  • To investigate the expression profile of lemma proteins and identify novel proteins.

Main Methods:

  • Protein extraction from wheat lemma at anthesis.
  • Peptide sequence analysis using polyvinylidene difluoride membranes.
  • Cleveland peptide mapping for internal sequence analysis of blocked proteins.

Related Experiment Videos

  • Expressed Sequence Tag (EST) database comparison.

    • Main Results:

    • Successfully identified and sequenced 48 out of 70 larger protein spots from wheat lemma.
    • Wheat proteins exhibited fewer N-terminal blocks compared to rice proteins.
    • Forty-one out of 56 analyzed amino acid sequences were assigned to corresponding ESTs.
    • The expression profile of lemma proteins was similar to leaf proteins, with most related to cellular metabolism.
    • One unique protein spot in lemma, absent in leaf, was identified.

    Conclusions:

    • This study provides the first proteomic characterization of wheat lemma at anthesis.
    • The findings suggest differences in N-terminal protein blocking between wheat and rice.
    • Lemma protein expression is largely conserved with leaf tissues, highlighting core metabolic functions.