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Related Experiment Videos

Transgenic mice in apoptosis research.

G Gil-Gómez1, H J Brady

  • 1Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. ggg@nki.nl

Apoptosis : an International Journal on Programmed Cell Death
|December 4, 2003
PubMed
Summary

Transgenic mice models are crucial for apoptosis research, offering insights into gene functions in primary cells and physiological processes. These studies advance understanding in areas from lymphoid development to neurodegeneration.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Apoptosis research is vital for understanding cell death mechanisms.
  • Studying apoptosis-related gene products in primary cells offers unique insights compared to cell lines.
  • Transgenic mouse models provide a powerful in vivo system for these investigations.

Purpose of the Study:

  • To review the utility of transgenic mice in apoptosis research.
  • To highlight insights gained from studying apoptosis-related gene products in vivo.
  • To explore the impact of these genes on physiological processes and disease models.

Main Methods:

  • Generation of transgenic mice expressing specific apoptosis-related molecules (e.g., Bcl-2 family, CD95).
  • Analysis of gene product function in primary cells derived from these mice.

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  • Assessment of physiological impacts across diverse biological systems.

    • Main Results:

    • Transgenic mice expressing various apoptosis regulators have been successfully created.
    • Studies reveal the distinct roles of apoptosis genes in primary cells versus cell lines.
    • Insights span lymphoid development, neurodegeneration, and intracellular signaling pathways.

    Conclusions:

    • Transgenic mice are indispensable tools for dissecting apoptosis mechanisms.
    • These models facilitate the study of gene function in complex physiological contexts.
    • Research using transgenic mice has significantly advanced our understanding of cell death and related diseases.