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Related Experiment Videos

UV-B irradiated cell lines execute programmed cell death in various forms.

M Hagenhofer1, H Germaier, C Hohenadl

  • 1Institute of Clinical Immunology and Rheumatology, Department of Medicine III, Friedrich-Alexander-University, Erlangen-Nuremberg, Erlangen, Germany.

Apoptosis : an International Journal on Programmed Cell Death
|December 4, 2003
PubMed
Summary

UV-B irradiation induces apoptosis markers in most cell lines, but not all. Some cells show atypical responses, highlighting the need for comprehensive apoptosis detection methods.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Apoptosis, or programmed cell death, is crucial for development and tissue homeostasis.
  • UV-B radiation is a known inducer of cellular damage and apoptosis.
  • Standard apoptosis detection methods may not capture all forms of programmed cell death.

Purpose of the Study:

  • To investigate UV-B induced apoptosis markers across diverse cell lines.
  • To evaluate the reliability of standard apoptosis assays in different cellular contexts.
  • To explore novel methods for DNA fragmentation analysis.

Main Methods:

  • UV-B irradiation of various cell lines.
  • Assessment of cellular morphology, phosphatidylserine (PS) exposure (annexin-V binding), DNA fragmentation (oligonucleosomal and high-molecular-weight), and nuclear changes (hypochromia, TUNEL assay).

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  • Development of a new method for isolating high-molecular-weight (hmwt) DNA fragments.

    • Main Results:

    • Most cell lines exhibited typical apoptosis markers like PS exposure, DNA fragmentation, and morphological changes after UV-B irradiation.
    • Raji and HaCaT cells showed no oligonucleosomal DNA fragmentation.
    • HaCaT cells displayed other apoptosis features, while Raji cells showed atypical DNA fragmentation and hypochromia but underwent growth arrest with PS exposure.

    Conclusions:

    • UV-B induced programmed cell death exhibits cell-type-specific variations.
    • Absence of oligonucleosomal DNA fragmentation or chromatin condensation does not exclude programmed cell death.
    • Comprehensive apoptosis detection is necessary due to diverse cellular responses.