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Updated: Aug 16, 2026

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
Published on: April 15, 2010
High-resolution 1H NMR spectroscopy in a live mouse subjected to 1.5 Hz magic angle spinning
Robert A Wind1, Jian Zhi Hu, Donald N Rommereim
1Pacific Northwest National Laboratory, Richland, Washington 99352, USA. robert.wind@pnl.gov
Abstract:
It is demonstrated that the resolution of the (1)H NMR metabolite spectrum in a live mouse can be significantly enhanced by an ultraslow magic angle spinning of the animal combined with a modified phase-corrected magic angle turning (PHORMAT) pulse sequence. Proton NMR spectra were measured of the torso and the top part of the belly of a female BALBc mouse in a 2 T field while spinning the animal at a speed of 1.5 Hz. It was found that even in this relatively low field, with PHORMAT an isotropic spectrum is obtained with line widths that are a factor of 4.6 smaller than those obtained in a stationary mouse. It is concluded that in vivo PHORMAT has the potential to significantly increase the utility of (1)H NMR spectroscopy for biochemical and biomedical animal research.
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