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Updated: Aug 30, 2026

Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
Published on: October 13, 2014
Effect of hypothalamic proline-rich-polypeptide on voltage-gated Ca2+ currents in retinal ganglion cells
1Department of Ophthalmology, New York University School of Medicine, New York, New York 10016, USA. aa3@nyu.edu
Abstract:
Neurotrophins are molecules that regulate neuronal survival, nervous system plasticity, and many other physiological functions of neuronal and glial cells. Here we studied the physiological action of a novel neurosecretory polypeptide proline-rich polypeptide (PRP), isolated from bovine neurohypophysis neurosecretory granules, on voltage-gated Ca currents and spike firing activity of retinal ganglion cells. PRP reversibly increased high voltage-activated L-type Ca current, but was without effect on low voltage-activated T-type current. PRP also increased the spike after hyperpolarization and reduced the frequency of spike firing, most likely by affecting a Ca-dependent potassium current.
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