Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

S-antigen localization in developing rds mouse retina.

S Sai1, J Usukura, T Shinohara

  • 1Department of Ophthalmology, Nagoya University, Japan.

Japanese Journal of Ophthalmology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of tumor burden and growth rate on treatment outcomes of nivolumab in head and neck cancer.

International journal of clinical oncology·2020
Same author

Hypoplastic left heart syndrome with regressed ventriculocoronary fistulae after the Norwood operation.

General thoracic and cardiovascular surgery·2019
Same author

Management of refractory chylothorax after pediatric cardiovascular surgery.

Pediatric cardiology·2012
Same author

Low glucose under hypoxic conditions induces unfolded protein response and produces reactive oxygen species in lens epithelial cells.

Cell death & disease·2012
Same author

Cerebral CT angiography using a small volume of concentrated contrast material with a test injection method: optimal scan delay for quantitative and qualitative performance.

The British journal of radiology·2012
Same author

Induction of apoptosis in head and neck tumor-cell lines by anti-fas antibody.

International journal of oncology·2011

In developing retinal degeneration slow (rds) mutant mice, S-antigen protein levels initially rise then fall, correlating with photoreceptor cell degeneration. This suggests protein loss may be a secondary effect of membrane malfunction in rds mice.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Genetics

Background:

  • The retinal degeneration slow (rds) mutant mouse is a model for inherited retinal diseases.
  • Understanding photoreceptor cell development and degeneration is crucial for treating blindness.

Purpose of the Study:

  • To investigate the morphology of photoreceptor cells and the localization of S-antigen in developing rds mutant mice.
  • To elucidate the role of S-antigen in the pathogenesis of retinal degeneration in rds mice.

Main Methods:

  • Improved immunocytochemical and freeze-substitution methods were employed.
  • Light and electron microscopy were used to analyze S-antigen localization and photoreceptor cell morphology.
  • Studies were conducted on rds mutant mice from 2 to 505 postnatal days.

Related Experiment Videos

Main Results:

  • S-antigen labeling was first observed at postnatal day 10 (light microscopy) and day 3 (electron microscopy).
  • S-antigen levels peaked around postnatal day 20 and declined to undetectable levels by day 505.
  • S-antigen was found throughout the photoreceptor cytoplasm and in vesicles budding from the membrane, with subsequent loss observed.

Conclusions:

  • Photoreceptor cell degeneration in rds mice may result from the secondary loss of soluble and membrane proteins due to membrane malfunction.
  • The gene defect in rds mice likely affects membrane components like peripherin or a 39 kDa protein, leading to progressive vision loss.