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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
[cDNAs cloning of SARS-CoV PUMC2 viral genome]
Zheng Fan1, Xin-yu Tan, Bin Yin
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Objective:
To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain.
Methods:
Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced.
Results:
The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained.
Conclusions:
These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.
