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Updated: Aug 30, 2026
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Published on: July 27, 2021
[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein]
Xin-yu Tan1, Zheng Fan, Hua-jin Wang
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 10005, China.
Objective:
To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.
Methods:
According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA.
Results:
Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was obtained.
Conclusions:
The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.
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