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[Study on mechanical compression regulating the proliferation of young growing rat masticatory myocyte in vitro]
1Department of Orthodontics, Oral Hospital of Guangdong Province, Guangzhou 510280, China.
Objective:
To establish an experimental model which was the masticatory myocyte culture and force stimulation in vitro and study the proliferation changes of cultured masticatory myocytes caused by mechanical compression in young rat.
Methods:
Flow cytometry (FCM) was used to examine the changes of cellular DNA content and cell cycles of cultured masticatory myocytes.
Results:
The DNA content in experimental groups was higher than that in control. After the compressive force was applied for 2 hours, the proliferation index (PI) in experimental group became higher than that in control. Under a continuous pressure for 4 hours, the PI in 2,000 mu strain group reached the maximum (48.9%) but the PI in 4,000 mu strain group reached the minimum (39.0%).
Conclusion:
The proliferation of masticatory myocytes from young rat increased under certain force and certain period of time, but decreased if the force applied was overloaded.
