Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A cell-adhesion pathway regulates intercellular communication during Dictyostelium development.

Kirsten Kibler1, Jessica Svetz, Tu-Lan Nguyen

  • 1Graduate Program in Developmental Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.

Developmental Biology
|December 4, 2003
PubMed
Summary

Cell adhesion genes comC, lagC, and lagD are crucial for Dictyostelium development. Disruptions impair sporulation, indicating a signaling pathway essential for multicellular organism development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Online tutorial on survival analysis for biomarker discovery.

PLoS computational biology·2026
Same author

Early cAMP signaling orchestrates single-cell synchronicity throughout Dictyostelium development.

Communications biology·2026
Same author

Extracellular matrix mediates circulating tumor cell clustering in triple-negative breast cancer metastasis.

Nature communications·2026
Same author

Mutual suppression between mutations in the Dictyostelium Greenbeard pathway restores wild-type development.

BMC genomics·2025
Same author

Detection of Clinically Relevant Monogenic Copy-Number Variants by a Comprehensive Genome-Wide Microarray with Exonic Coverage.

Clinical chemistry·2025
Same author

Going against the family: Perturbation of a greenbeard pathway leads to falsebeard cheating.

iScience·2024

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Genetics

Background:

  • Cell adhesion molecules are vital for multicellular organism structure and signaling.
  • Intercellular communication genes play roles in development.
  • Understanding signaling pathways is key to developmental processes.

Purpose of the Study:

  • To elucidate a novel cell adhesion pathway involving comC, lagC, and lagD in Dictyostelium development.
  • To investigate the roles of these genes in cell signaling and sporulation.
  • To characterize the genetic network governing cell adhesion and development.

Main Methods:

  • Genetic analysis of comC, lagC, and lagD mutants in Dictyostelium.
  • Developmental assays using pure populations and chimeras.

Related Experiment Videos

  • Transcriptional and functional analyses of gene interactions.

    • Main Results:

    • Mutants lacking comC, lagC, or lagD exhibit impaired sporulation in isolation but rescue in chimeras with wild-type cells.
    • Pairwise chimeras of mutants fail to sporulate, suggesting a shared signaling deficiency.
    • Evidence supports a regulatory network where comC influences lagC and lagD, and lagC/lagD mutually induce each other.

    Conclusions:

    • The genes comC, lagC, and lagD function in a conserved signaling pathway essential for Dictyostelium sporulation.
    • Wild-type cells provide a signal that rescues mutant sporulation defects.
    • The cell adhesion gene lagC acts as a terminal component in this developmental signaling network.