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Updated: Feb 11, 2026

Collection of Frozen Rodent Brain Regions for Downstream Analyses
Published on: April 23, 2020
The microsomal calcium-ATPase inhibitor thapsigargin is a neurotoxin in perinatal rodent brain
1Department of Pediatrics, University of Michigan, Ann Arbor 48109.
Abstract:
Thapsigargin is a potent inhibitor of the microsomal calcium-ATPase. We hypothesized that thapsigargin-induced liberation of calcium from sequestered intracellular sites could result in irreversible brain injury. To assess neurotoxicity, intracerebral injections of 0.1-100 nmol thapsigargin were performed in 7-day-old rats, and the severity of injury was assessed 5 days later by microscopy and measurements of lesioned areas. We found that thapsigargin elicited dose-dependent tissue injury in perinatal rodent brain.
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