Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The bag method for islet cell infusion.

David A Baidal1, Tatiana Froud, Jacqueline V Ferreira

  • 1Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136, USA.

Cell Transplantation
|December 5, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two decades of advancing safe and effective cell-based therapies: a review of the impact, promise, and current limitations of USA regulation 21 CFR Part 1271.

Cytotherapy·2026
Same author

Injury-specific effect of Schwann cell-derived exosome treatment for peripheral nerve injury.

PloS one·2026
Same author

Diagnostic Accuracy, Implementation Barriers, and Equity Implications of Teledermatology in Rural Skin Cancer: Scoping Review.

JMIR dermatology·2025
Same author

Design and rationale of CATO, a Phase IIA, randomized, double-blind, placebo-controlled study of single or repeated intravenous administration of umbilical cord-derived mesenchymal stromal cells in ischemic cardiomyopathy.

American heart journal·2025
Same author

Phase I Randomized Study of Cardiac Stem Cells in Patients With Hypoplastic Left Heart Syndrome: The CHILD Trial.

JACC. Heart failure·2025
Same author

Acute Pleuropericardial Syndrome Following Messenger Ribonucleic Acid (mRNA) COVID-19 Vaccination: A Case Report.

Cureus·2025
Same journal

Transplantation of human umbilical mesenchymal stromal cells attenuates heart failure progression in a rat model.

Cell transplantation·2026
Same journal

Three-dimensionally printed mesoporous bioactive glass for craniomaxillofacial bone regeneration: Material evolution, functional mechanisms, and clinical translation.

Cell transplantation·2026
Same journal

Hotspots and trends in stem cell therapy for ligament injuries: A bibliometric analysis (2001-2025).

Cell transplantation·2026
Same journal

Amniotic epithelial cells enhance islet engraftment by suppressing early inflammation in intraportal transplantation.

Cell transplantation·2026
Same journal

Cdc42-Modified BMSC-Derived exosomes promote acellular nerve allografts to bridge sciatic nerve defects.

Cell transplantation·2026
Same journal

Organ-on-a-chip as a next-generation tool in drug development: A bibliometric and patent analysis.

Cell transplantation·2026
See all related articles

A novel gravity-fed system for islet cell transplantation offers a safer alternative to syringe methods. This technique helps prevent dangerous portal pressure increases and portal vein thrombosis during transplantation.

Area of Science:

  • Transplantation immunology
  • Surgical procedures
  • Gastroenterology

Background:

  • Islet cell transplantation is gaining interest, necessitating standardized procedures.
  • Previous methods using syringe infusion have been linked to complications like portal vein thrombosis.
  • Reproducible results from the Edmonton group highlight the need for procedural guidelines.

Purpose of the Study:

  • To detail a standardized, safe, and effective closed gravity-fed bag system for islet cell infusion.
  • To present a method that minimizes complications observed with syringe-based infusion techniques.

Main Methods:

  • Utilizing a closed gravity-fed system with a 600-ml transfer bag and a 150-ml rinse bag connected via sterile tubing.
  • Employing gravity for controlled infusion rates, which naturally reduces flow with increased portal pressure.

Related Experiment Videos

  • Implementing this technique at an institution since 1990.

    • Main Results:

    • The gravity-fed method demonstrated no instances of partial or complete portal vein thrombosis in performed infusions.
    • This technique allows for controlled infusion rates, preventing precipitous portal pressure rises.
    • The method has been adopted by most centers performing clinical islet transplantation.

    Conclusions:

    • The closed gravity-fed bag system provides a safe and effective method for islet cell transplantation.
    • This technique helps mitigate risks such as portal hypertension and portal vein thrombosis.
    • Standardization of islet infusion procedures is crucial for successful transplantation outcomes.