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Inhibitory effect of quercetin on proliferation of human microvascular endothelial cells in vitro
Pan-Sheng Fan1, Zhen-Lun Gu, Rui Sheng
1Department of Pharmacology, Institute of Chinese Materia Medica, Soochow University, Suzhou 215007, China.
Aim:
To investigate the role of quercetin (Que) in the proliferation of cultured human skin microvascular endothelial cells (MVEC).
Methods:
Cell count and [methyl-3H]thymidine ([3H]TdR) uptake assay were used to measure the effect of Que in the proliferation of cultured MVEC. Cytotoxicity of Que on MVEC was also evaluated by 51Cr release assay.
Results:
When MVEC were treated with Que, the proliferation was significantly inhibited in a time-course and dose-dependent manner. Que 5 micromol/L did not inhibit the proliferation of MVEC. When the concentration of Que increased to 20, 40, 80, and 160 micromol/L, the cell numbers per well were decreased and the inhibition rate was 12.2 %, 23.5 %, 35.3 %, and 54.1 % respectively with IC50 of 138 micromol/L. The inhibitory rate of [3H]-TdR uptake was 18.7 %, 34.4 %, 48.9 %, and 62.5 % respectively (IC(50)=87.5 micromol/L). (51)Cr release assay showed that Que 160 micromol/L incubated with MVEC from 1 to 16 h had no clear cytotoxicity compared with control group.
Conclusion:
Que greatly inhibited the proliferation of cultured human MVEC in vitro. This effect may not be related to the cytotoxicity of Que on MVEC.
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