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Updated: Aug 29, 2026
Horizontal Hippocampal Slices of the Mouse Brain
Published on: September 22, 2020
Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons
Guang-Qin Zhang1, Xue-Mei Hao, Shi-Zhong Chen
1State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Aim:
To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice.
Methods:
The whole-cell patch clamp technique was used.
Results:
Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal I(Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I(Na) inactivation state from (4.2+/-0.7) ms to (9.8+/-1.2) ms.
Conclusion:
Pae inhibited I(Na) in mouse hippocampus neurons.
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