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Updated: Aug 29, 2026
Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
Published on: January 11, 2019
Identification and quantification of free radicals during myocardial ischemia and reperfusion using electron
Catherine Vergely1, Véronique Maupoil, Gaelle Clermont
1Laboratoire de Physiopathologie et Pharmacologie Cardiovasculaires Expérimentales, IFR no 100-Facultés de Médecine et de Pharmacie, 7 Bd Jeanne d'Arc-87900, 21079 Dijon Cedex, France.
Abstract:
There is general agreement that free radicals are involved in reperfusion injury. Electron paramagnetic resonance (EPR) spectroscopy can be considered as the more suitable technique to directly measure and characterize free radical generation during myocardial ischemia and reperfusion. There are essentially two approaches used in the detection of unstable reactive species: freezing technique and spin traps. The detection of secondary free radicals or ascorbyl free radicals during reperfusion might provide an index of oxidative stress. Spin trapping can also characterize nitric oxide. EPR spectroscopy can provide important data regarding redox state and free radical metabolism but ideally, the spin traps must not interfere with cell or organism function.