Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Roc, a Ras/GTPase domain in complex proteins.

Leonard Bosgraaf1, Peter J M Van Haastert

  • 1Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

Biochimica Et Biophysica Acta
|December 5, 2003
PubMed
Summary

Researchers discovered a new protein domain, Roc, within the Ras/GTPase superfamily. This Roc domain, always paired with a COR domain, is found in diverse organisms from bacteria to animals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Local Ras/actin signaling orients individual pseudopods in shallow gradients.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Collaboration of Antipodes: Synergy of Branched and Linear F-Actin during Amoeboid Cell Movement and Chemotaxis.

Cold Spring Harbor perspectives in biology·2025
Same author

Pseudopod Tracking and Statistics During Cell Movement in Buffer and Chemotaxis.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

GRminusRD: A Sensitive Assay to Detect Activation Processes at the Plasma Membrane in Living Cells.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Analysis of cGMP Signaling in Dictyostelium.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Forty-five years of cGMP research in <i>Dictyostelium</i>: understanding the regulation and function of the cGMP pathway for cell movement and chemotaxis.

Molecular biology of the cell·2021

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • The Ras/GTPase superfamily plays crucial roles in cellular signaling.
  • Understanding novel protein domains is key to deciphering complex biological functions.

Purpose of the Study:

  • To identify and characterize a previously unknown protein domain within the Ras/GTPase superfamily.
  • To investigate the structural context and evolutionary distribution of this novel domain.

Main Methods:

  • Bioinformatic analysis of protein sequences.
  • Identification of conserved protein domains.
  • Comparative genomics across different species.

Main Results:

  • A novel Ras/GTPase domain, termed Roc, was identified.
  • The Roc domain is consistently associated with a novel C-terminal domain, termed COR.
  • Roc/COR domains are found in a wide range of organisms, including prokaryotes, Dictyostelium, plants, and metazoa.
  • Roc domains are integrated into larger proteins with diverse functional domains like LRRs, ankyrin repeats, WD40 repeats, kinase domains, RasGEF, and RhoGAP.

Conclusions:

  • The discovery of the Roc/COR domain expands the known repertoire of the Ras/GTPase superfamily.
  • The widespread distribution suggests significant functional importance in diverse biological processes.
  • The modular nature of Roc/COR-containing proteins indicates versatile roles in cellular regulation.

Related Experiment Videos