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Preparation and use of a monoclonal antibody to detect Chlamydia psittaci antigen in paraffin-embedded tissue
1Università degli Studi di Torino, Dipartimento di Patologia Animale, Italy.
Research in Veterinary Science
|November 1, 1992
Abstract:
A murine monoclonal antibody prepared against an ovine abortion isolate of Chlamydia psittaci (A22/Teramo) revealed specific binding to a 57 kDa chlamydial antigen in immunoblotting studies. The monoclonal antibody was able to detect intracytoplasmic chlamydial inclusions and scattered elementary bodies in infected McCoy cell culture, and on formalin-fixed paraffin-embedded tissue sections both from experimentally infected mice and from fetal membranes of cases of ovine enzootic abortion.
