Related Experiment Video
Updated: Aug 29, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Continuous D-tagatose production by immobilized thermostable L-arabinose isomerase in a packed-bed bioreactor
Se-Ah Ryu1, Chang Sup Kim, Hye-Jung Kim
1Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
Abstract:
D-Tagatose was continuously produced using thermostable L-arabinose isomerase immobilized in alginate with D-galactose solution in a packed-bed bioreactor. Bead size, L/D (length/diameter) of reactor, dilution rate, total loaded enzyme amount, and substrate concentration were found to be optimal at 0.8 mm, 520/7 mm, 0.375 h(-1), 5.65 units, and 300 g/L, respectively. Under these conditions, the bioreactor produced about 145 g/L tagatose with an average productivity of 54 g tagatose/L x h and an average conversion yield of 48% (w/w). Operational stability of the immobilized enzyme was demonstrated, with a tagatose production half-life of 24 days.
Related Concept Videos
Upstream Processing
Production of Antibiotics
Production of Alcohol
Production of Pharmaceuticals
Bioreactor Design and Operational System
Production of Organic Acids
