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Related Experiment Videos

Determination of bopindolol using the flow injection technique coupled with solid phase extraction.

Joana Meireles1, Hana Sklenárová, Dalibor Satínský

  • 1CEQUP/Department of Physical Chemistry, Faculty of Pharmacy, University of Porto, Rua Aníbal Cunha 164, 4050, Porto, Portugal.

Journal of Pharmaceutical and Biomedical Analysis
|December 6, 2003
PubMed
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A new flow injection analysis (FIA) method determines bopindolol in tablets using spectrophotometry and solid-phase extraction (SPE). This automated technique offers accurate and efficient bopindolol quantification with high sample throughput.

Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Analysis

Background:

  • Bopindolol is a beta-blocker used to treat hypertension.
  • Accurate quantification of bopindolol in pharmaceutical formulations is crucial for quality control.

Purpose of the Study:

  • To develop and validate a flow injection analysis (FIA) method for bopindolol determination.
  • To utilize solid-phase extraction (SPE) for pre-concentration and improved sensitivity.

Main Methods:

  • A lab-made automated FIA system with spectrophotometric detection at 635 nm was employed.
  • Bopindolol was complexed with ferric ions in an acidic medium.
  • Solid-phase extraction (SPE) using a mini-column integrated into the flow system was used for pre-concentration.

Main Results:

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  • The SPE-FIA method achieved a seven-fold pre-concentration of bopindolol.
  • A sample throughput of 50 samples per hour was obtained.
  • The method demonstrated a linear range of 125-1000 µg/mL with an R.S.D. of 1.87% and a detection limit of 70 µg/mL.

Conclusions:

  • The developed SPE-FIA method provides a reliable and efficient approach for bopindolol determination in Sandonorm tablets.
  • Results showed good agreement with a conventional High-Performance Liquid Chromatography (HPLC) method.