Related Experiment Video
Updated: Aug 29, 2026
Murine Dermal Fibroblast Isolation by FACS
Published on: January 7, 2016
Flow-cytometric DNA analysis in cutaneous fibrohistiocytic tumors
Hiroshi Okita1, Tsutomu Ohtsuka, Soji Yamazaki
1Department of Dermatology, Dokkyo University School of Medicine, Mibu, Tochigi, Japan.
Background:
Fibrohistiocytic tumors are rare skin tumors. There are many kinds of fibrohistiocytic tumors. It is often difficult to differentiate dermatofibrosarcoma protuberans (DFSP) and malignant fibrous histiocytoma (MFH) from dermatofibroma (DF) and other benign tumors.
Objective:
In this study, the possible usefulness of DNA ploidy for the differentiation was studied.
Methods:
Five MFH, 9 DFSP, 3 DF, 2 lipofibromas and 2 scars were studied for DNA ploidy using a flow cytometer.
Results:
All the 7 benign tumors showed a diploid pattern. Of 14 malignant fibrohistiocytic tumors, 8 cases (57.1%) showed an aneuploid and 6 a diploid pattern. Two of 5 MFH (40.0%) and 6 of 9 DFSP (66.7%) showed an aneuploid pattern. These results showed that fibrohistiocytic tumors with an aneuploid pattern are malignant, though those with a diploid pattern may be benign or malignant.
Conclusion:
Analysis of DNA ploidy using flow cytometry was concluded to be useful for the differentiation of malignant fibrohistiocytic tumors from benign ones.
More Related Videos
09:14Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
Published on: May 7, 2019
07:43Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Published on: July 29, 2017
