Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time-resolved Fourier transform infrared spectroscopic imaging.

Rohit Bhargava1, Ira W Levin

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0510, USA.

Applied Spectroscopy
|December 9, 2003
PubMed
Summary

This study introduces time-resolved infrared spectroscopic imaging for observing rapid molecular changes in materials. This advanced technique enables simultaneous spatial and temporal chemical measurements of dynamic processes occurring in milliseconds.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting human mRNA isoform levels from site-specific splicing kinetics <i>in silico</i>.

bioRxiv : the preprint server for biology·2026
Same author

Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.

APL photonics·2026
Same author

Survivor Perspectives on Artificial Intelligence Integration in Breast Cancer Treatment: A Qualitative Study of Trust, Equity, and Application.

European journal of breast health·2026
Same author

Cytologic Detection of Metastatic Immature Teratomas in Two Young Patients With Unique Presentations.

Diagnostic cytopathology·2026
Same author

Determination of an optimal quality control method for Pap test analysis using digital cytology and artificial intelligence.

Journal of the American Society of Cytopathology·2026
Same author

Non-invasive detection of local microstructural damage in tendon using Diffusion Tensor MRI.

Acta biomaterialia·2026

Area of Science:

  • Spectroscopy
  • Materials Science
  • Chemical Imaging

Background:

  • Fourier transform infrared (FT-IR) imaging offers broad spatial analysis but limited temporal resolution.
  • Existing methods restrict the study of dynamic processes to those with minute-scale timescales.
  • Investigating rapid molecular dynamics requires enhanced temporal resolution in spectroscopic techniques.

Purpose of the Study:

  • To introduce a time-resolved infrared spectroscopic imaging modality.
  • To enable the examination of dynamic processes with millisecond-scale half-lives.
  • To demonstrate simultaneous spatial and temporal chemical measurements.

Main Methods:

  • Development and implementation of a step-scan FT-IR imaging technique.
  • Application to a polymer-liquid crystal composite subjected to electric-field perturbations.

Related Experiment Videos

  • Analysis using univariate and generalized two-dimensional (2D) correlation methods.

    • Main Results:

    • Achieved millisecond-level temporal resolution for infrared spectroscopic imaging.
    • Successfully captured molecular responses to external electric-field perturbations.
    • Demonstrated the capability for simultaneous spatial and temporal chemical analysis of dynamic molecular processes.

    Conclusions:

    • The developed time-resolved FT-IR imaging modality significantly enhances the study of rapid molecular dynamics.
    • This technique provides unprecedented insights into the spatiotemporal behavior of materials.
    • It opens new avenues for investigating dynamic chemical processes in various fields.